Generator

Part:BBa_K766005:Design

Designed by: Yu Zhao   Group: iGEM12_Tsinghua   (2012-09-24)


T7 promoter-Hig tag-LuxI wihout LVA tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 67
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BamHI site found at 755
    Illegal XhoI site found at 750
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 67
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We want to build a signal sender which is easy detected and controled.


Source

We clone this gene from our lab's plamid which is commonly used

References