Generator
Part:BBa_K766005:Design
Designed by: Yu Zhao Group: iGEM12_Tsinghua (2012-09-24)
T7 promoter-Hig tag-LuxI wihout LVA tag
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 67
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
Illegal BamHI site found at 755
Illegal XhoI site found at 750 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 67
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 67
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We want to build a signal sender which is easy detected and controled.
Source
We clone this gene from our lab's plamid which is commonly used